1. M. Shinohara, H. Arakawa, Y. Oda, N. Shiraki, S. Sugiura, T. Nishiuchi, T. Satoh, K. Iino, S. Leo, Y. Kato, K. Araya, T. Kawanishi, T. Nakatsuji, M. Mitsuta, K. Inamura, T. Goto, K. Shinha, W. Nihei, K. Komori, M. Nishikawa, S. Kume, Y. Kato, T. Kanamori, Y. Sakai, H. Kimura, "Coculture with hiPS‑derived intestinal cells enhanced human hepatocyte functions in a pneumatic‑pressure‑driven two‑organ microphysiological system", Scientific reports, vol.11, 5437, 2021. [link]
  2. T. Matsumura, T. Sato, T. Abe, H. Sanjo, K. Katagiri, H. Kimura, T. Fujii, H. Tanaka, M. Hirabayashi, T. Ogawa, "Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control", Scientific reports, vol.11, 3458, 2021. [link]


  1. S. Mise, S. Shibagaki, S. Nishikawa, H. Nakamura, H. Kimura, *A. Takamatsu, "Diversity in self-organized forms and migration modes in isolated epithelial cells", Artificial Life and Robotics, vol.25, pp.523-528, 2020. [link]
  2. Y. Ikeda, P. Jurica, H. Kimura, H. Takagi, Z.R. Struzik, K. Kiyono, Y. Arata, Y. Sako, "C. elegans episodic swimming is driven by multifractal kinetics", Scientific Reports, vol.10,14775, 2020. [link]
  3. K. Shinha, W. Nihei, *H. Kimura, "A microfluidic probe integrated device for spatiotemporal 3D chemical stimulation in cells", Micromachines, vol.11, no.7, 691, 2020. [link]
  4. K. Shinha, W. Nihei, T. Ono, R. Nakazato, *H. Kimura, "A pharmacokinetic-pharmacodynamic model based on multi-organ-on-a-chip for drug-drug interaction studies", Biomicrofluidics, vol.14, no.4, 44108, 2020. [link]
  5. M. Chikamori, H. Kimura, R. Inagi, J. Zhou, M. Nangaku, *T. Fujii, "Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress", Biomicrofluidics, vol.14, no.4, 44102, 2020. [link]
  6. D. Kasahara, H. Sumiyoshi, H. Endo, T. Yanagawa, Y. Nakano, Y. Matsuki, S. Nakao, A. Kamiya, H. Kimura, *Y. Inagaki, "Visualization and Isolation of Zone-Specific Murine Hepatocytes That Maintain Distinct Cytochrome P450 Oxidase Expression in Primary Culture", Biochemical and Biophysical Research Communications, vol.528, no.3, pp.420-425, 2020. [link]
  7. K. Sasaki, K. Bandai, M. Takatsuka, M. Fujii, M. Takagi, R. Kita, S. Yagihara, H. Kimura, N. Shinyashiki, "Heterogeneous solvent dielectric relaxation in polymer solutions of water and alcohols", Frontiers in Physics, vol.8, no.84, 2020. [link]
  8. H. Zhang, D. Sakagami, W. Huang, H. Kimura, Y. Okamura, "Measurement and modelling of tensile moduli of polymer blend thin films with phase separated structures", Polymer, vol.190, 122233, 2020. [link]
  9. Arata Y, Oshima T, Ikeda Y, Kimura H, Sako Y, "OP50, a bacterial strain conventionally used as food for laboratory maintenance of C. elegans, is a biofilm formation defective mutant", microPublication Biology, 2020. [link]


  1. M. Komeya, H. Yamanaka, H. Sanjo, M. Yao, H. Nakamura, H. Kimura, T. Fujii, T. Sato, *T. Ogawa, "In vitro spermatogenesis in two-dimensionally spread mouse testis tissues", Reproductive Medicine and Biology, vol.18, no.4, pp.362-369, 2019. [link]
  2. S. Yokoyama, A. Otomo, S. Hadano, *H. Kimura, "An open-type microdevice to improve the quality of fluorescence labeling for axonal transport analysis in neurons", Biomicrofluidics, vol.13, no.3, 34104, 2019. [link]
  3. M. Nishikawa, S. Yuri, H. Kimura, N. Yanagawa, M. Hamon, P. Hauser, L. Zhao, OD Jo, N. Yanagawa, "Comprehensive analysis of chromatin signature and transcriptome uncovers functional lncRNAs expressed in nephron progenitor cells", Biochimica et Biophysica Acta – Gene Regulatory Mechanisms, vol.1862, no.1, pp.58-70, 2019. [link]


  1. T. Ohkubo, H. Kinoshita, T. Maekawa, K. Kunida, H. Kimura, S. Kuroda, T. Fujii, "Development of a microfluidic cell culture and monitoring system for intracellular signaling studies", bioRxiv, 453100, 2018. [link]
  2. T. Hiraiwa, Y. Nakai, T. G Yamada, R. Tanimoto, H. Kimura, Y. Matsumoto, N. Miki, N. Hiroi, A. Funahashi, "Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole‐to‐pole axis of mitotic cells by using a microfluidic device", FEBS Open Bio, vol.8, no.12, PP1920-1935, 2018. [link]
  3. W. Huang, Y. Sunami*, H. Kimura, S. Zhang, "Applications of Nanosheets in Frontier Cellular Research", Nanomaterials, vol.8, no.7, 519, 2018. [link]
  4. K. Kojima, H. Nakamura, M. Komeya, H. Yamanaka, Y. Makino, Y. Okada, T. Sato, T. Fujii, H. Kimura, T. Ogawa, "Neonatal testis growth recreated in vitro by two-dimensional organ-spreading", Biotechnology and Bioengineering, vol.115, no.12, pp.3030-3041, 2018 [link]
  5. M. Nishikawa, H. Kimura, N. Yanagawa, M. Hamon, P. Hauser, L. Zhao, OD Jo, N. Yanagawa, "An optimal serum-free defined condition for in vitro culture of kidney organoids", Biochemical and Biophysical Research Communications, vol.501, no.4, pp.996-1002, 2018. [link]
  6. H. Kimura, M. Nishikawa, N. Yanagawa, H. Nakamura, S. Miyamoto, M. Hamon, P. Hauser, L. Zhao, O. D. Jo, M. Komeya, T. Ogawa, N. Yanagawa, "Effect of fluid shear stress on in vitro cultured ureteric bud cells in a pumpless microfluidic device", Biomicrofluidics, vol.12, no.4, 44107, 2018. [link]
  7. H. Yamanaka, M. Komeya, H. Nakamura, H. Sanjo, T. Sato, M. Yao, H. Kimura, T. Fujii, T. Ogawa, "A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility", Biochemical and Biophysical Research Communications, vol.500, no.4, pp.885-891, 2018. [link]
  8. K. Haneda, T. Oishi, H. Kimura, T. Inazu, "Development of a microbioreactor for glycoconjugate synthesis", Bioorganic & Medicinal Chemistry, vol.26, no.8, pp.2092-2098, 2018. [link]
  9. H. Kimura, Y. Sakai, T. Fujii, "Organ/Body-on-a-chip Based on Microfluidic Technology for Drug Discovery", Drug Metabolism and Pharmacokinetics, vol.33, no.1, pp.43-48, 2018. [link]


  1. M. Komeya, K. Hayashi, H. Nakamura, H. Yamanaka, H. Sanjo, K. Kojima, T. Sato, M. Yao, H. Kimura, T. Fujii, T. Ogawa, "Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro", Scientific Reports, vol.7, no.1, 15459, 2017. [link]
  2. 高橋翼, 中村寛子, 木村啓志, "精子選別機能集積型受精卵作出デバイスの開発",日本機械学会論文集, vol.83, no.850, 16, 2017. [link]
  3. 宮本隼佑, 亀崎勇暁, 諸星和, 喜多理王, 木村啓志, "ルードヴィヒ・ソレー効果を利用した重水分離システムの構築", 化学とマイクロ・ナノシステム学会会誌, vol.16, no.1, pp.47-48, 2017.
  4. Y. Nakano, S. Sachie, H. Sumiyoshi, K. Mikami, Y. Tanno, M. Sueoka, D. Kasahara, H. Kimura, T. Moro, A. Kamiya, K. Hozumi and Y. Inagaki, "Identification of a Novel Alpha-Fetoprotein-Expressing Cell Population Induced by Jagged1/Notch Signal in Murine Fibrotic Liver", Hepatology Communications, vol.1, no.3, pp.215-229, 2017. [link]
  5. K. M. Ganesh, M. Morohoshi, Y. Yasoda, S. Yokoyama, H. Kimura, K. Tsuchiya, "ZnO Based Microfluidic pH Sensor: A Versatile Approach for Quick Recognition of Circulating Tumor Cells in Blood", ACS Applied Materials & Interfaces, vol.9, no.6, pp.5193-5203, 2017. [link]


  1. Horayama, K. Shinha, K. Kabayama, T. Fujii, H. Kimura, "Spatial Chemical Stimulation Control in Microenvironment by Microfluidic Probe Integrated Device for Cell-Based Assay", PLOS ONE, vol.11, no.12, 2016. [link]
  2. M. Komeya, H. Kimura, H. Nakamura, T. Yokonishi, T. Sato, K. Kojima, K. Hayashi, K. Katagiri, H. Yamanaka, H. Sanjo, M. Yao, S. Kamimura, K. Inoue, N. Ogonuki, A. Ogura, T. Fujii, T. Ogawa, "Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device", Scientific Reports, vol.6, 21472, 2016. [link]


  1. L. Yang, Y. Okamura, H. Kimura, "Surface Modification on Polydimethylsiloxane-based Microchannels with Fragmented Poly(L-lactic acid) Nanosheets", Biomicrofluidics, vol.9, 064108, 2015. [link]
  2. H. Kimura, T. Ikeda, H. Nakayama, Y. Sakai, T. Fujii, "An On-chip Small Intestine-Liver Model for Pharmacokinetic Studies", Journal of Laboratory Automations, vol.20, no.3, pp.265-273, 2015. [link]
  3. H. Tozawa, T. Maekawa, H. Kimura, T. Fujii, "A novel effect of parylene-based surface coating on HepG2 cell function", Materials Science and Engineering C, vol.46, pp.190-194, 2015. [link]


  1. P.V. Hauser, M. Nishikawa, H. Kimura, T. Fujii, N. Yanagawa, "Controlled Tubulogenesis from Dispersed Ureteric Bud-derived Cells Using a Micropatterned Gel", Journal of Tissue Engineering and Regenerative Medicine, vol.10, no.9, pp.762-771, 2014. [link]
  2. H. Nakayama, H. Kimura, T. Fujii, Y. Sakai, "Image-based evaluations of distribution and cytotoxicity of Irinotecan (CPT-11) in a multi-compartment micro-cell coculture device", J. Biosci. Bioeng., vol.117(6), pp.756–762, 2014. [link]
  3. M. Shinohara, H. Kimura, K. Montagne, K. Komori, T. Fujii, Y. Sakai, "Combination of microwell structures and direct oxygenation enables efficient and size-regulated aggregate formation of an insulin-secreting pancreatic beta-cell line", Biotechnol. Prog., vol.30(1), pp.178-187, 2014. [link]


  1. X. He, H. Kimura, T. Fujii, "A High-Throughput Device for Patterned Differentiation of Embryoid Bodies", Journal of Robotics and Mechatronics, vol.25, no.4, pp.623-630, 2013. [link]


  1. J. Kawada, H. Kimura, H. Akutsu, Y. Sakai, T. Fujii, "Spatiotemporally controlled delivery of soluble factors for stem cell differentiation", Lab on a Chip, vol.12, no.21, pp.4508-4515, 2012. [link]
  2. M.M. Chowdhury, H. Kimura, T. Fujii, Y. Sakai, "Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4", Biomicrofluidics, vol.6, 014117, 2012
  3. K. Komori, S. Fujii, K. Montagne, H. Nakamura, H. Kimura, T. Otake, T. Fujii, Y. Sakai "Development of a Well-of-the-Well System-Based Embryo Culture Plate with an Oxygen Sensing Photoluminescent Probe", Sensors and Actuators B, vol.162, pp.278-283, 2012
  4. T. Nojima, S. Kaneda, H. Kimura, T. Yamamoto, T. Fujii, "Application of Cell-free Expression of GFP for Evaluation of Microsystems", Frontiers in Bioscience, vol.17, pp.1931-1939, 2012


  1. Y. Nakao, H. Kimura, Y. Sakai, T. Fujii, "Bile canaliculi formation by aligning rat primary hepatocyte in a microfluidic device", Biomicrofluidics, vol.5, pp.022212-1-7, 2011
  2. T. Kurakazu, K. Kuribayashi-Shigetomi, Y. T. Matsunaga, H. Kimura, T. Fujii, Y. Sakai, S. Takeuchi, "Selective Retrieval Method Using Electrolytically Generated Bubbles for Cell Array Applications", Sensors and Actuators B, vol.159, pp.229-233, 2011


  1. H. Kimura, H. Takeyama, K. Komori, T. Yamamoto, Y. Sakai, T. Fujii, "Microfluidic Device with Integrated Glucose Sensor for Cell-based Assay in Toxicology", Journal of Robotics and Mechatronics, vol.22, no.5, pp.594-600, 2010 [link]
  2. 木村啓志, 庄野裕基, Pereira-Rodrigues, 山本貴富喜, 酒井康行, 藤井輝夫, "オンチップグルコースセンサによる細胞活性オンライン計測の検討", 電気学会論文誌E, vol.130, no.10, pp.476-483, 2010. [link]
  3. M.M. Chowdhury, T. Katsuda, K. Montagne, H. Kimura, K. Nobuhiko, H. Akutsu, T. Ochiya, T. Fujii, Y. Sakai, "Enhanced effects of secreted soluble factor preserve better pluripotent state of embryonic stem cell culture in a membrane-based compartmentalized micro-bioreactor", Biomed Microdevices, vol.12, no.6, pp.1097-1105, 2010. [link]


  1. H. Kimura, H. Nakamura, T. Akai, T. Yamamoto, H. Hattori, Y. Sakai, T. Fujii, "On-chip Individual Embryo Coculture with Microporous Membrane-supported Endometrial Cells", IEEE Transactions on NanoBioscience, vol.8, no.4, pp.318-324, 2009(Chemical Biology (2008, issue 6)にも掲載). [link]
  2. 木村啓志, 中山秀謹, 山本貴富喜, 酒井康行, 藤井輝夫, "体内毒性試験へ向けたオンチップ小腸-肝臓由来細胞共培養システムの開発", 電気学会論文誌E, 129, no.8, pp.252-258, 2009. [link]
  3. 木村啓志, 中村寛子, 岩井孝介, 山本貴富喜, 竹内昌治, 藤井輝夫, 酒井康行, "ダイナミックマイクロアレイを用いた受精卵操作の自動化の試み", 電気学会論文誌E, 129, no.8, pp.245-251, 2009. [link]


  1. T. Nojima, T. Yamamoto, H. Kimura, T. Fujii, "Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription/ translation of green fluorescent protein as a report gate", Chemical Communications, vol.28, no.32, pp.3771-3773, 2008. [link]
  2. T. Nojima, H. Kimura, T. Fujii, "Cell-free Protein Synthesis Conducted by Template DNA with Repetitive Sequence", Chemistry Letters, vol.37, no.6, pp.648-649, 2008. [link]
  3. H. Kimura, T. Yamamoto, H. Sakai, Y. Sakai, T. Fujii, "An Integrated Microfluidic System for Long-term Perfusion Culture and On-line Monitoring of Intestinal Tissue Models", Lab on a Chip, vol.8, no.5, pp.741-746, 2008, May. [link]


  1. H. Kimura, M. Nishikawa, T. Yamamoto, Y. Sakai, T. Fujii, "Microfluidic Perfusion Culture of Human Hepatocytes", Journal of Robotics and Mechatronics, vol.19, no.5, pp.550-556, 2007. [link]
  2. H. Nakayama, H. Kimura, K. Komori, T. Fujii Y. Sakai, "Development of a Multi-Compartment Micro-Cell Culture Device as a Future on-Chip Human: Fabrication of a Three-Compartment Device and Immobilization of Rat Mature Adipocytes for the Evaluation of Chemical Distributions", Journal of Robotics and Mechatronics, vol.19, no.5, pp.544-549, 2007. [link]